Pontificia Universidad Católica de Chile

Systems
Bioengineering
Laboratory

Our group builds computational tools rooted in advanced optimization and machine learning to redesign and understand biological systems.

Metabolic Modeling Systems Biotechnology Bioprocess Optimization Machine Learning

Research Lines

02

Systems Biotechnology

Engineering biological systems for sustainable production of food ingredients and high-value chemicals.

Related papers
03

Bioprocess Optimization

Advanced optimization frameworks for bioreactor design, control, and process scale-up.

Related papers
04

Machine Learning

Computational statistics and ML techniques to infer, predict, and design biological behavior from sparse data.

Related papers
05

Open-Source Tools

Publicly released computational tools — METACONE, GRASP, LooplessFluxSampler, Fast-SNP, ll-ACHRB, among others.

Explore software

Systems BioEngineering Lab

PS

Prof. Pedro A. Saa

Group Leader

Faculty member at the Department of Chemical and Bioprocess Engineering and the Institute for Mathematical and Computational Engineering of PUC Chile. Interests include genome-scale network reconstruction and analysis, metabolic modelling and engineering, and bioprocess and biosystems optimization.

Metabolic Engineering Biosystems Modelling Optimization Computational Statistics Applied Machine Learning

Education

PhD — The University of Queensland, 2017 MEng — PUC Chile, 2012 BEng — PUC Chile, 2012
NameYearsCurrent Position
María Catalina Casanueva 2022–2025 Process Engineer, CCU — Chile
Ricardo Leiva 2021–2025 Software Engineer, DICTUC SA — Chile
Benjamín Elizondo 2021–2024 Bioprocess Engineer, Advanced Biotech — NJ, US
Sebastián Zapararte 2022–2024 AI Engineer, Sticta Biologicals — Chile
Carolina Deantas 2021–2023 PhD Student, ITQB Nova — Portugal

Articles

All Papers

2024
2022

Mendoza S, Saa PA, Teusink B, Agosin E

Metabolic modelling of wine fermentation at genome scale

Methods in Molecular Biology vol. 2399, Springer

Under Review

Vargas-Luna C, Tapia I, Godoy L, Saa PA, Franco W.

Characterization and kinetic modeling of Levilactobacillus brevis malolactic fermentation under oenological conditions.

Under Review

Sepúlveda-Celis A, Agosin E, Pérez-Correa JR, Saa PA.

A computational framework for building dynamic bioreactor models integrating detailed enzyme kinetics with limited data: beta-ionone production in S. cerevisiae.

Under Review

Tapia I, Torrealba C, Luna R, Pérez-Correa JR, Saa PA.

Dynamic balance of sparse flux vectors for efficient simulation of culture dynamics and network reduction.

Under Review

Torrealba C, Luna R, Saa PA, Pérez-Correa JR.

A systematic transfer workflow for robust model identification across scales: Application to wine fermentation.

Under Review

In Preparation

Zapararte S, Marcellin E, Nielsen LK, Saa PA.

Topologically-constrained sampling of thermodynamically feasible mass-balanced states in metabolic reaction networks.

In Preparation

Saa PA, Miranda G, Drovandi C, Nielsen LK.

Bayesian parameter inference on the simplex: Convenient transformations and perturbation kernels for Sequential Monte Carlo sampling.

In Preparation

Resources

GitHub Lab

News & Milestones

2026

Official lunch of VitiScience

Our group leader was part of the official lunch of VitiScience at the Center for Research and Innovation of Viña Concha y Toro in Pencahue, Talca.

2026

New FONDECYT Regular ANID Grant Awarded — Principal Investigator

Grant No. 1262500 — Scalable prediction of metabolic fluxes using modelling frameworks informed by enzyme kinetics and omics data (2026–2029).

2026

New Paper: Beer Digital Twin

Congrats Colomba! Our group recently published in Journal of Food Engineering — a digital model integrating industrial process data for beer quality control.

2026

New Paper: METACONE released

Congrats Álvaro! Our group just published METACONE in Computational Biology and Chemistry — a scalable framework for computing metabolic conversions.

2025

New Avanza UC Grant Awarded — Principal Investigator

Grant No. AV25292 for optimizing EPS production in Sulfobacillus acidophilus using metabolic engineering.

2025

New ANID Center for Applied Research established

Joined the Center for Research and Innovation VitiScience as Adjunct Investigator.

2025

New Paper: Kinetic model of β-carotene in yeast

Congrats Benjamín! We just published a detailed kinetic model predicting β-carotene production in ACS Synthetic Biology.

2024

New Paper: LooplessFluxSampler released

We have recently published an efficient loopless flux sampling algorithm in BMC Bioinformatics.

Active Projects

Principal Investigator

Basal Center for Artificial Intelligence Research

2021–ongoing

Principal Investigator

FONDECYT Regular No. 1262500 — Scalable prediction of metabolic fluxes informed by enzyme kinetics and omics data.

2026–2029

Co-Investigator

FONDECYT Exploración No. 13250023 — Impact of GABA-producing bacterial consortia in neuroprotection

2025–2029

Co-Investigator

FONDECYT Regular No. 1230764 — Model-guided analysis of microbial interactions in gut microbiota

2023–2027

Principal Investigator

Avanza UC AV25292 — EPS production from mining waste via S. acidophilus

2026–2027

Adjunct Investigator

Center for Research and Innovation VitiScience

2025–ongoing

Join the Lab

Our lab uses both wet and dry lab approaches to better understand biological systems. We welcome motivated students and researchers excited about developing new computational approaches for rediscovering biology. We have openings for domestic and international applicants at all levels.

Contact Prof. Pedro Saa directly at pnsaa@uc.cl  ·  +56 2 2354 9674